| 1 |  Arif KovacPenalty goals: 1 |  Atlanta United 2 | 12 |
| 2 | Alex KrehlPenalty goals: 0 |  Chattanooga FC | 10 |
| 2 | Zach ZenguePenalty goals: 0 |  Columbus Crew 2 | 10 |
| 4 | Marcus CaldeiraPenalty goals: 1 |  Minnesota United 2 | 10 |
| 5 | Austin BrummettPenalty goals: 0 |  Houston Dynamo 2 | 8 |
| 5 |  Barnabás TanyiPenalty goals: 0 |  Connecticut United | 8 |
| 5 | Edouard NysPenalty goals: 0 |  North Texas SC | 8 |
| 5 |  Kevin GbambléPenalty goals: 0 | Columbus Crew 2 | 8 |
| 5 |  Nathaniel JamesPenalty goals: 0 | North Texas SC | 8 |
| 5 |  Nimfasha BerchimasPenalty goals: 0 |  Crown Legacy FC | 8 |
| 5 | Palmer AultPenalty goals: 0 |  St. Louis City 2 | 8 |
| 5 |  Rodolfo AlokoPenalty goals: 0 | Crown Legacy FC | 8 |
| 13 |  Maximus EkkPenalty goals: 1 |  Huntsville City FC | 8 |
| 14 |  Hugo MbonguePenalty goals: 3 | Crown Legacy FC | 8 |
| 15 |  Chase AdamsPenalty goals: 0 | Columbus Crew 2 | 7 |
| 15 | Dylan VanneyPenalty goals: 0 |  Ventura County FC | 7 |
| 15 | Idoh Zeltzer-ZubidaPenalty goals: 0 |  Inter Miami CF II | 7 |
| 18 |  Jorge AlastueyPenalty goals: 2 |  Austin FC II | 7 |
| 18 |  Pedro LeãoPenalty goals: 2 |  Orlando City B | 7 |
| 20 |  Tate RobertsonPenalty goals: 3 | Chattanooga FC | 7 |
| 21 |  Arnaud TattevinPenalty goals: 0 |  Carolina Core FC | 6 |
| 21 | Daniel IttycheriaPenalty goals: 0 |  Vancouver Whitecaps 2 | 6 |
| 21 |  Darius RandellPenalty goals: 0 | Minnesota United 2 | 6 |
| 21 |  Julian DonneryPenalty goals: 0 |  San Jose Earthquakes II | 6 |
| 21 |  Tega IkobaPenalty goals: 0 |  Sporting Kansas City II | 6 |
| 21 |  Tommy MihalicPenalty goals: 0 |  Los Angeles FC II | 6 |
| 21 | Zachary BohanePenalty goals: 0 | San Jose Earthquakes II | 6 |
| 28 |  Damien Barker JohnPenalty goals: 1 | Chattanooga FC | 6 |
| 28 | Jahmarie NolanPenalty goals: 1 |  Toronto FC II | 6 |
| 28 |  Julian PlaciasPenalty goals: 1 | Ventura County FC | 6 |
| 28 | Myles MorganPenalty goals: 1 |  New England Revolution II | 6 |
| 32 | Daniel CervantesPenalty goals: 2 |  Portland Timbers 2 | 6 |
| 33 | Cooper FlaxPenalty goals: 3 |  New York City FC II | 6 |
| 34 |  Alex McGrathPenalty goals: 0 | Chattanooga FC | 5 |
| 34 |  Cameron DunbarPenalty goals: 0 | Atlanta United 2 | 5 |
| 34 | Dennis NelichPenalty goals: 0 |  Red Bull New York II | 5 |
| 34 |  Eddy Davis IIIPenalty goals: 0 |  Philadelphia Union II | 5 |
| 34 | Harvey SarajianPenalty goals: 0 | Orlando City B | 5 |
| 34 | Kashan HinesPenalty goals: 0 | Sporting Kansas City II | 5 |
| 34 | Lineker RodriguesPenalty goals: 0 |  Real Monarchs SLC | 5 |
| 34 |  Mark BronnikPenalty goals: 0 |  Tacoma Defiance | 5 |
| 34 | Nathan RichmondPenalty goals: 0 | Crown Legacy FC | 5 |
| 34 |  Sebastiano MusuPenalty goals: 0 | New York City FC II | 5 |
| 34 |  Stas KorzeniowskiPenalty goals: 0 | Philadelphia Union II | 5 |
| 45 |  Yu TsukanomePenalty goals: 1 | Tacoma Defiance | 5 |
| 46 |  Alex MonisPenalty goals: 2 | Connecticut United | 5 |
| 46 |  Stefan ChirilaPenalty goals: 2 |  FC Cincinnati 2 | 5 |
| 48 |  Liam MackenziePenalty goals: 3 | Vancouver Whitecaps 2 | 5 |
| 49 | Benjamin RodriguezPenalty goals: 0 | Red Bull New York II | 4 |
| 49 | Daniel D'IppolitoPenalty goals: 0 | Connecticut United | 4 |
